Methods in Protein Biochemistry eBook
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By applying techniques to pursue rapid kinetic reactions it is possible to separate a complete reaction into different time segments and to determine the rate constants of individual steps of the reaction. The author demonstrates the methods by which this is done and explains the theory behind it. Topics include reversible enzyme reactions, enzyme inhibition, multi-substrate reactions, special enzyme mechanisms, and isotope exchange. Specific methods detailed include electrochemical methods, calorimetry, and spectroscopy.
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